Liquid-liquid extraction (LLE) is a highly effective technique for extracting compounds in bioanalysis. The LLE method involves transferring an analyte of interest between an immiscible organic solvent and an aqueous phase. To selectively extract desired analytes with sufficient recovery, a large volume of immiscible organic solvents like hexane, MTBE, or Ethyl Acetate are commonly used in LLE methods.
Download our poster that was presented at WRIB 2024, to see how we modified the LLE method to notably improve the assay’s efficiency and performance.
Authors:
Jayaprakash (JP) Kotha, MBBS, PhD, ASCP (SH)
Vice President, Bioanalytical Laboratory
Leimin (Perry) Fan, PhD
Associate Director, Method Development
Alicia Bian
Method Development Scientist, Principal Research Scientist
